edgeOF

This function will flag objects on the edge of wells that are often more difficult to segment properly with CellProfiler.

Usage

edgeOF(data, radius = 825, center_x = 1024, center_y = 1024)

Arguments

data

A data frame output from the modelSelection function.

radius

Radius in pixels away from image center with even illumination. The default value is set to 825, which is standard for images taken with the imageXpress nano 2X objective.

center_x

center x position of image in pixels. Default is 1024.

center_y

center y position of image in pixels. Default is 1024.

Value

A single data frame with edge_ObjectFlag and well_edge_flag_radius

variables added. The edge_ObjectFlag variable is coded as "edge" for objects with centroids outside the radius provided, all other objects are coded as NA_character.