Protocols
C. elegans Phenotyping Methods
COPAS BIOSORT
DNA Methods
- Yeast-mediated ligation plasmid construction
- 96-Well DNAPrep - Omega Bio-tek
- 96-Well High-multiplexing Tagmentation and Amplification
- Generating Libraries for Whole-Genome Sequencing
- Qiagen Gentra DNA preparation for C. elegans
- DNAEasy Genomic Prep
- 96-Well Quantification Of DNA
- 96-Well DNAPrep - Gentra Purgene
- Synthetic sgRNA
- High Throughput Genotyping for Wild Isolate Identification
- CTAB DNA Prep of A. dissimilis
- CRISPR-Cas9 Genome Editing by RNP Injection
- DNAEasy Genomic Prep - QiaCube
General C. elegans
- EMS mutagenesis
- Superbroth and HB101 growth
- Methanol/Acetone fixation for Whole-mount staining
- Chunking Worms
- Flow Chart For A Cross
- Single Worm Genotyping
- Microinjection
- Cleaning Worms
- Soft Agar Freezing and Thawing
- How to Build a NIL
- Synchronizing Worms by Bleaching
- Crude Dauer Pheromone Prep
- Growing OP50 Cultures for Seeding Plates
- Seeding NGMA Plates With Bacteria
- Bleaching and Dispensing of C. elegans using the BioTek MicroFlo Select
- Andersen Lab Strain Intake
- Worm Maintenance
- Identifying New Wild Isolates
- Andersen Lab Nematode Field Sampling Protocol
- Nematode Collection Protocol
- Worm Collecting Protocol - Citizen Scientist
- 15-hour Bacterial Food Growth
- Cryopreservation and DNA Preps of Caenorhabditis Nematodes
- Steps to Freeze Wild Isolate DNA Samples